#!/usr/bin/env perl use strict; use warnings; use FindBin; use lib ("$FindBin::Bin/../../PerlLib"); use Process_cmd; my $usage = "\n\n\tusage: $0 Trinity.fasta reads.fa [threads=1]\n\n"; my $trin_fa = $ARGV[0] or die $usage; my $reads_fa = $ARGV[1] or die $usage; my $CPU = $ARGV[2] || 1; main: { my $salmon_index = "$trin_fa.salmon.idx"; my $salmon_stderr = "_salmon.$$.stderr"; my $cmd = "salmon --no-version-check index -t $trin_fa -i $salmon_index -k 25 -p $CPU > $salmon_stderr 2>&1"; &run_cmd_capture_stderr($cmd, $salmon_stderr); $cmd = "salmon --no-version-check quant -i $salmon_index -l U -r $reads_fa -o salmon_outdir -p $CPU --minAssignedFrags 1 --validateMappings > $salmon_stderr 2>&1"; &run_cmd_capture_stderr($cmd, $salmon_stderr); unlink($salmon_stderr); exit(0); } #### sub run_cmd_capture_stderr { my ($cmd, $stderr_file) = @_; eval { &process_cmd($cmd); }; if ($@) { my $errmsg = `cat $stderr_file`; die "Error, cmd: $cmd failed with msg: $errmsg $@"; } return; }