#!/usr/bin/env perl use strict; use warnings; use Carp; use FindBin; use lib ("$FindBin::RealBin/../../PerlLib"); use Fasta_reader; use Ktree; use Nuc_translator; my $usage = "usage: $0 fasta_file KmerSize [DSmode]\n\n"; my $fasta_file = $ARGV[0] or die $usage; my $kmer_length = $ARGV[1] or die $usage; my $DS_mode = $ARGV[2] || 0; main: { my $ktree = new Ktree(); my $fasta_reader = new Fasta_reader($fasta_file); while (my $seq_obj = $fasta_reader->next()) { my $seq = $seq_obj->get_sequence(); my @chars = split(//, $seq); for (my $i = 0; $i <= length($seq) - $kmer_length; $i++) { my $kmer = join("", @chars[$i..($i+$kmer_length-1)]); $ktree->add_kmer($kmer); if ($DS_mode) { $ktree->add_kmer( &reverse_complement($kmer) ); } } } $ktree->report_kmer_counts(); exit(0); }