Add scripts for phylogenetic analysis using MrBayes and RAxML
- Created `mbblock.txt` for MrBayes configuration. - Implemented `mrbayes.sh` to convert FASTA to Nexus and run MrBayes. - Developed `raxml.sh` to run modeltest-ng and raxml-ng on alignments. - Added `04.filter_ogs.sh` to filter orthologous groups based on taxon count and sequence length. - Implemented `01.run_mrbayes.sh` to execute MrBayes for multiple FASTA files. - Created `02.mbsum.sh` to summarize MrBayes output while skipping high DSF analyses. - Developed `03.run_raxml.sh` to run raxml-ng on filtered alignments.
This commit is contained in:
@@ -0,0 +1,20 @@
|
||||
#! /bin/bash
|
||||
set -e
|
||||
SCRIPTS=${SCRIPTS:-"$PROJECTHOME/99.scripts"}
|
||||
|
||||
if [ "$#" -ne 2 ]; then
|
||||
echo "Usage: $0 <input_fasta> <output_nexus>"
|
||||
exit 1
|
||||
fi
|
||||
|
||||
in_fasta=$(readlink -f "$1")
|
||||
out_nex=$2
|
||||
|
||||
# Convert fasta to nexus
|
||||
out_dir=$(dirname "$out_nex")
|
||||
mkdir -p "$out_dir"
|
||||
seqmagick convert --output-format nexus --alphabet dna --input-format fasta "$in_fasta" "$out_nex"
|
||||
# append MrBayes block to nexus file
|
||||
cat "$SCRIPTS"/miscs/mbblock.txt >>"$out_nex"
|
||||
# Run MrBayes
|
||||
mb "$out_nex" >"${out_dir}"/mrbayes.log 2>&1
|
||||
Reference in New Issue
Block a user